| 1. | A method to construct 12 kb plasmid vector
重组质粒载体的方法 |
| 2. | Second round pcr products were cloned to t / a plasmid vectors to set up the subtractive library
Westernblot分析了nadp一苹果酸酶蛋白合成随盐胁迫的变化情况。 |
| 3. | Rynai - w and xynb1 were all cloned into the plasmid vector ppic9 q , and were all overexpressed in pichia pastoris gs l 1 5
将xynb1序列克隆进酵母表达载体ppic9上，在毕赤酵母gs115中得到高效特异性表达。 |
| 4. | Result , we succeed in doing t - pa gene from fetal lung and construct a sort of eukaryon expression plasmid vector with pcdna3 . 1 ( + )
结果：成功地从胎儿肺组织中克隆了t - pa基因，并构建了以pcdna3 . 1 ( + )为载体的真核表达质粒载体。 |
| 5. | Put this fusion gene under the control of the promoter of ie1 gene , and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted
将该融合基因置于ie1基因启动子的控制之下，构建成了真核表达质粒pgem ie1 - gfp - actin 。 |
| 6. | In order to construct plant artificial chromosome , plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms
为了构建植物人工染色体，我们构建了可以和含有着丝粒序列的yac载体的左臂和右臂进行同源重组的质粒载体。 |
| 7. | Using a pair of special primers , whole sequence of m gene of b95 were amplified by rt - pcr and be sequenced after being directly inserted in plasmid vector pbluescript sk
结果表明： b95m蛋白基因全长1232bp ，共编码364个氨基酸，其中碱性氨基酸有47个，酸性氨基酸有30个，等电点为9 |
| 8. | Furthermore , we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine , compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors
此外，我们还探讨了诱导前免疫致敏对t细胞疫苗活性的影响，并比较了hcv腺病毒载体和质粒载体在此免疫致敏功能方面的差异。 |
| 9. | It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0 . 5 - 3kb fragments of a . brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors
Brasilensesp7的nifa全序列构建在pgbd - c2载体上，得到重组质粒pgbd - nifa ，以其为诱饵，筛选sp7质粒基因组文库(构建在pgad系列质粒上) 。 |
| 10. | Two primers were desighed and synthesized . xynbj coding nature protein of xynb have been cloned by pcr . then xynd1 - s nyna ] - w and xynb1 were all cloned into the plasmid vector pet - 22b ( + ) , and were all expressed in e coli
将此序列克隆进表达载体pet - 22b ( + )上，在大肠杆菌bl21 ( de3 )中也得到特异性表达，并有正常的生物学活性，证明了基因xynb1的生物学功能。 |
